An example of DNA sequencing from a research based paper by me from Zhejiang Ocean University

Check this out...

DNA sequencing

COI amplification used the pair of primers, FishF1 and FishR1(Ward et al., 2005). PCR was carried out in Thermo Cycler with a 25 μL volumes, containing 8.5 μL sterile nuclease free water, 2 μL of each primer , 12.5 μL Go Taq® Green Master Mix (Promega Inc, U.S.A.) and 2 μL of template DNA. PCR program used a thermal profile, including a preliminary denaturation for 2 minutes at 95 °C followed by 35 cycles of denaturation at 95 °C for 40 seconds, annealing at 52°C for 40 seconds, and extension at 72°C for 90 seconds, and finally a single extra extension at 72°C for 5 minutes. PCR products were confirmed via gel electrophoresis using 1.5% agarose gel stained with SybrGreen fluorescent dye for band characterization through Gel Imaging System. Subsequently, PCR products were sent for sequencing at Sangon Biotech Cooperation Ltd (Shanghai, P.R. China)

Comments

Xiamen University Scholarships

APPLY NOW Xiamen University in China offers a range of scholarship programs for international students who wish to study there. Here are some of the scholarship programs available: FULLY FUNDED: Australia Awards Scholarships (Deadline: 1 May 2023 (annual)) Xiamen University Scholarships for New International Students: This scholarship is awarded to outstanding international students who are applying for undergraduate or graduate programs at Xiamen University. The scholarship covers tuition fees, accommodation fees, and a monthly stipend. >Chinese Government Scholarship: This scholarship is awarded to international students who wish to pursue a master's or doctoral degree at Xiamen University. The scholarship covers tuition fees, accommodation fees, and a monthly stipend. >Xiamen University Tan Kah Kee Scholarships: These scholarships are awarded to international students from Southeast Asia who wish to study at Xiamen University. The scholarship covers tuition fees, accommoda...

Wafahamu Matajiri 20 Duniani

Wafuatao ni orodha ya matajiri 20 duniani, wakwanza wao akiwa ni Jeff Bezos, mmiliki wa Amazon mwenye thamani ya utajiri wa Dola za kimarekani Bilioni 131 akifuatiwa na Bill Gates mwenye utajiri wa thamani ya Dola za kimarekani 96.5 muanzishaji wa kampuni ya Microsoft na mmiliki wa Bill na Melinda Gates foundation. 1. Jeff Bezos $131B 2. Bill Gates $ 96.5 B 3. Warren Buffett $82.5 B 4. Bernard Arnault &family $76 B 5. Carlos Slim Helu & family $64 B 6. Amancio Ortega $62.7 B 7. Larry Ellison $ 62.5 B 8. Mark Zuckerberg $62.3 B 9. Michael Bloomberg $55.5 B 10. Larry Page $50.8B 11. Charles Koch $50.5B 11. David Koch $50.5B 13. Mukesh Ambani $50B 14. Sergey Brin $49.8 B 15. Francoise Bettencourt Meyers & family $49.3B 16. Jim Walton $44.6 B 17. Alice Walton $44.4 B 18. Rob Walton $44.3B 19. Steve Ballmer $41.2 B 20. Ma Huateng $38.8 B Sources: Forbes

39th International Coral Reef Initiative General Meeting

* ICRI Members --> SAVE THE DATE * 20-22 April 2026 for the 39th ICRI General Meeting in JEDDAH For any enquiries, please contact the Secretariat icri@shams.gov.sa #nafasimpyazakazi #daresalaamjobs #newjobsopportunities #jobstanzania #newopportunities #newemploymentopportunities #hiringnow #scholarshipsopportunities #fullyfundedscholarships #fullyfunded #mabumbe #ajiraleo #ajiraportal #ajiratz #brightermonday #cvpeople #jobshunting #jobsinarusha #jobsindar #jobsinmbeya #jobsinmwanza #jobsindodoma #chinascholarships #chinascholarshipsopportunities #ajiraportaltz

Easy Opportunities

Search This Blog