An example of DNA sequencing from a research based paper by me from Zhejiang Ocean University

Check this out...

DNA sequencing

COI amplification used the pair of primers, FishF1 and FishR1(Ward et al., 2005). PCR was carried out in Thermo Cycler with a 25 μL volumes, containing 8.5 μL sterile nuclease free water, 2 μL of each primer , 12.5 μL Go Taq® Green Master Mix (Promega Inc, U.S.A.) and 2 μL of template DNA. PCR program used a thermal profile, including a preliminary denaturation for 2 minutes at 95 °C followed by 35 cycles of denaturation at 95 °C for 40 seconds, annealing at 52°C for 40 seconds, and extension at 72°C for 90 seconds, and finally a single extra extension at 72°C for 5 minutes. PCR products were confirmed via gel electrophoresis using 1.5% agarose gel stained with SybrGreen fluorescent dye for band characterization through Gel Imaging System. Subsequently, PCR products were sent for sequencing at Sangon Biotech Cooperation Ltd (Shanghai, P.R. China)

Comments

QUOTE OF THE DAY

why should you visit mkomazi national park? 🦏

Mkomazi National Park is located in northeastern Tanzania on the Kenyan border, in Kilimanjaro Region and Tanga Region. It was established as a game reserve in 1951 and upgraded to a national park in 2006. Mkomazi National Park has a truly spectacular wilderness! It provides a common base for migratory herds and has a classic arid areas. The park covers over 3,234 square kilometres (323,400 ha), and is dominated by Acacia-Commiphora vegetation; it is contiguous with Kenya's Tsavo West National Park. The area commonly called 'Mkomazi' is actually the union of two previous game reserves, the Umba Game Reserve in the east (in Lushoto District, Tanga Region) and the Mkomazi Game Reserve in the west (in Same District, Kilimanjaro Region); in government documents they are sometimes called the Mkomazi-Umba Game Reserves. Of the two, Mkomazi is larger, and has more diversity of relief and habitat, and a longer shared border with Tsavo West National Park. In the rest of this entry, ...

Xiamen University Scholarships

APPLY NOW Xiamen University in China offers a range of scholarship programs for international students who wish to study there. Here are some of the scholarship programs available: FULLY FUNDED: Australia Awards Scholarships (Deadline: 1 May 2023 (annual)) Xiamen University Scholarships for New International Students: This scholarship is awarded to outstanding international students who are applying for undergraduate or graduate programs at Xiamen University. The scholarship covers tuition fees, accommodation fees, and a monthly stipend. >Chinese Government Scholarship: This scholarship is awarded to international students who wish to pursue a master's or doctoral degree at Xiamen University. The scholarship covers tuition fees, accommodation fees, and a monthly stipend. >Xiamen University Tan Kah Kee Scholarships: These scholarships are awarded to international students from Southeast Asia who wish to study at Xiamen University. The scholarship covers tuition fees, accommoda...

Easy Opportunities

Search This Blog