An example of DNA sequencing from a research based paper by me from Zhejiang Ocean University

Check this out...

DNA sequencing

COI amplification used the pair of primers, FishF1 and FishR1(Ward et al., 2005). PCR was carried out in Thermo Cycler with a 25 μL volumes, containing 8.5 μL sterile nuclease free water, 2 μL of each primer , 12.5 μL Go Taq® Green Master Mix (Promega Inc, U.S.A.) and 2 μL of template DNA. PCR program used a thermal profile, including a preliminary denaturation for 2 minutes at 95 °C followed by 35 cycles of denaturation at 95 °C for 40 seconds, annealing at 52°C for 40 seconds, and extension at 72°C for 90 seconds, and finally a single extra extension at 72°C for 5 minutes. PCR products were confirmed via gel electrophoresis using 1.5% agarose gel stained with SybrGreen fluorescent dye for band characterization through Gel Imaging System. Subsequently, PCR products were sent for sequencing at Sangon Biotech Cooperation Ltd (Shanghai, P.R. China)

Comments

TANGAZO LA KUITWA KWENYE USAILI MAJINA YA NYONGEZA

TANGAZO LA KUITWA KWENYE USAILI MAJINA YA NYONGEZA 05/04/2024

Fully Funded Nanyang President’s Graduate Scholarship in Singapore 2022 (Application Open)

The Nanyang President’s Graduate Scholarship (NPGS) is a competitive and prestigious scholarship scheme designed to encourage outstanding graduates or final-year students to take their first step towards a leading research career by studying for a full time PhD at NTU. Eligibility There is no restriction as to the nationality of candidates. Preference will be given to Singapore Citizens and Singapore Permanent Residents. You must have a Honours (Highest Distinction) or equivalent at Bachelor’s level. If you have not yet completed your undergraduate degree programme, you will need to furnish documentation from your university that you are on track to get a Honours (Highest Distinction) degree or equivalent. Coverage and Award Terms There is no bond attached to the scholarship. The award is tenable for one year in the first instance and is renewable subject to good progress. The maximum period of the Scholarship is 4 years for PhD candidates, as determined by school as well a...

New Job Opportunities at Sokoine University of Agriculture February 2022

The Management of Sokoine University of Agriculture (SUA) invites suitable qualified Tanzanians to apply for various positions. The applicotion letter should indicate names and addresses of three referees, together with certified copies of all academic certificates. POSITION: LIVESTOCK FIELD TECHNICIAN I (ONE POSITION) – College of Veterinary Medicine and Biomedical Sciences QUALIFICATIONS: Holder of Diploma in one of the following fields: Animal Health, Animal Health and Production, or equivalent qualifications from a recognized Institution with working experience of at least two (2) years in Artificial Insemination. DUTIES AND RESPONSIBILITIES: To carry out Artificial Insemination to the Animal hospital clients and SUA farms, To assist in Identifying and selecting suitable clinical cases for teaching, To assist in identifying primary reproduction disorders in animals, To perform any other related duties as may be assigned by the supervis...

Easy Opportunities

Search This Blog