An example of DNA sequencing from a research based paper by me from Zhejiang Ocean University

Check this out...

DNA sequencing

COI amplification used the pair of primers, FishF1 and FishR1(Ward et al., 2005). PCR was carried out in Thermo Cycler with a 25 μL volumes, containing 8.5 μL sterile nuclease free water, 2 μL of each primer , 12.5 μL Go Taq® Green Master Mix (Promega Inc, U.S.A.) and 2 μL of template DNA. PCR program used a thermal profile, including a preliminary denaturation for 2 minutes at 95 °C followed by 35 cycles of denaturation at 95 °C for 40 seconds, annealing at 52°C for 40 seconds, and extension at 72°C for 90 seconds, and finally a single extra extension at 72°C for 5 minutes. PCR products were confirmed via gel electrophoresis using 1.5% agarose gel stained with SybrGreen fluorescent dye for band characterization through Gel Imaging System. Subsequently, PCR products were sent for sequencing at Sangon Biotech Cooperation Ltd (Shanghai, P.R. China)

Comments

What are the 15 Most Dangerous Animals in Africa?

Africa is home to some of the world’s most powerful and dangerous wildlife. While most wild animals avoid humans, certain species can become dangerous when threatened, surprised, or approached too closely. Here are 15 animals known for causing serious injuries or posing significant risks to people. 1. 🦛 Hippopotamus Despite its slow appearance, the hippopotamus can be extremely aggressive, especially near water. It has powerful jaws and large canine teeth and can seriously injure or kill people. 2. 🐊 Nile Crocodile The Nile crocodile is a powerful ambush predator. It can attack animals and people who get too close to the water’s edge. 3. 🐘 African Elephant Elephants are generally peaceful when left undisturbed, but they can become dangerous when threatened, particularly females protecting calves and males during periods of heightened aggression. 4. 🦁 African Lion As Africa’s largest big cat, lions have the strength and hunting ability to kill large prey. Although attacks o...

10 Amazing Facts About African Elephants You Probably Didn’t Know

African elephants are among the most fascinating animals on Earth. From their incredible memory to their complex social lives, these giants play an important role in Africa’s ecosystems. Here are 10 amazing facts about African elephants you may not know. 1. African elephants are the world’s largest land animals The African bush elephant ( Loxodonta africana ) is the largest living land animal. Adult males can weigh several tonnes and stand more than 3 metres tall at the shoulder. 2. Their ears help them stay cool African elephants have large ears filled with blood vessels. By moving their ears, they help release body heat, an important adaptation to Africa’s hot climate. 3. Elephants use their trunks for many things An elephant’s trunk is used for breathing, smelling, touching, drinking, feeding and communication. It contains tens of thousands of muscles, giving it remarkable strength and flexibility. 4. They communicate in ways humans cannot always hear Elephants pr...

10 Interesting Facts About Giraffes

This photo was taken at Katavi National Park, Tanzania, Credits to Diana. Here are the 10 facts about Giraffes that you want to know, the source of these facts is at the bottom of this post. 1. Giraffes are the tallest mammals on Earth. Their legs alone are taller than many humans—about 6 feet. 2. They can run as fast as 35 miles an hour over short distances, or cruise at 10 mph over longer distances. 3. A giraffe's neck is too short to reach the ground. As a result, it has to awkwardly spread its front legs or kneel to reach the ground for a drink of water. 4. Giraffes only need to drink once every few days. Most of their water comes from all the plants they eat. 5. Giraffes spend ...

WILDLIFE TANGANYIKA

Search This Blog